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Submitted: 10 Nov 2021
Accepted: 10 Feb 2022
ePublished: 19 Feb 2022
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Immunopathol Persa. 2022;8(2): e31369.
doi: 10.34172/ipp.2022.31369

Scopus ID: 85133672380
  Abstract View: 1680
  PDF Download: 1116

Original

Ki-67 proliferative index in immunoglobulin A nephropathy; A pilot study

Maedeh Barahman 1 ORCID logo, Elham Emami 2 ORCID logo, Maryam Biglari Abhari 3 ORCID logo, Elham Ahmadian 4 ORCID logo, Muhammed Mubarak 5 ORCID logo, Mohammad Bahadoram 6 ORCID logo, Ahmad Reza Maghsoudi 7* ORCID logo, Azar Baradaran 8* ORCID logo, Niloufar Hooshyar 7,9 ORCID logo

1 Department of Radiation Oncology, Firoozgar Hospital, Firoozgar Clinical Research Development Center (FCRDC), Iran University of Medical Sciences (IUMS), Tehran, Iran
2 Pediatric Nephrology Research Center (PNRC), Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Emam Hosein Hospital, Tehran, Iran
3 Preventive Medicine and Public Health Research Center, Psychosocial Health Research institute, Community and Family Medicine Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
4 Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5 Sindh Institute of Urology and Transplantation (SIUT), Karachi, Pakistan
6 Thalassemia and Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
7 Department of Internal Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
8 Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
9 Nickan Research Institute, Isfahan, Iran
*Corresponding Authors: *Correspondence to Ahmadreza Maghsoudi, Email: ahmaghsoudi@med.mui.ac.ir, , Email: dr_maghsoudi_md@yahoo.com; *Correspondence to Prof. Azar Baradaran, Email; azarbaradaran@yahoo. com, , Email: azarbaradaran@med.mui.ac.ir

Abstract

Introduction: IgA nephropathy (IgAN) regarded as the main type of primary glomerulonephritis globally. It has a diverse clinical course and unpredictable prognosis. Traditionally, clinical and pathological features are conducted to prognosticate the outcome of the disease. Recently, attention has been directed towards other indices that may have prognostic implications for IgAN progression. The Ki-67 index have been extensively detected as a proliferation marker of malignant cells for several years.

Objectives: In this study, we aimed to determine the prognostic impact of Ki-67 expression in IgAN biopsies.

Patients and Methods: This cross-sectional study was conducted on 18 biopsy-proven IgAN patients. IgAN was diagnosed by significant IgA deposits and according to the morphologic variables of Oxford (MEST) classification for this disease. The expression of Ki-67 in the glomeruli, interstitium and tubules was described as the intensity percentage of the immunohistochemical staining.

Results: In all of the eighteen cases with biopsy-proven IgAN, the mean ± SD of age, degree of proteinuria and serum creatinine were 36.3±17 years, 1600±733 mg/day and 1.2±0.4 mg/dL, respectively. In this study, we found a significant correlation between glomerular Ki-67 immunohistochemical staining and MEST scores (r: 0.780, P< 0.001). We also detected a significant correlation between interstitial Ki-67 staining and MEST scores (r: 0.700, P=0.001). Moreover, Ki-67 immunohistochemical staining of the interstitium had a significant relationship with IgM deposits (r=0.544, P=0.02).

Conclusion: In this pilot study, we found that interstitial and glomerular Ki-67 immunohistochemical staining had positive and significant associations with MEST scores. Our data also showed that interstitial Ki-67 staining had a significant relationship with IgM deposits. These preliminary results require further investigation in large-scale studies.


Citation: Barahman M, Emami E, Biglari Abhari M, Ahmadian E, Mubarak M, Bahadoram M, Maghsoudi AR, Baradaran A, Hooshyar N. Ki-67 proliferative index in immunoglobulin A nephropathy; A pilot study. Immunopathol Persa. 2022;8(2):e31369. DOI:10.34172/ ipp.2022.31369.
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