Nickan Research Institute Immunopathologia Persa 2423-8015 4 2 2018 07 01 Antimicrobial effects of amniotic membrane on some bacterial strains e25 e25 10.15171/ipp.2018.25 EN Siamak Heidarzadeh Abdolmajid Ghasemian Zohreh Kalafi Farhad Nikkhahi Mohammad Mehdi Soltan Dallal Journal Article 10.15171/ipp.2018.25 2018 01 13 2018 05 06 Introduction: Antibiotic resistance in clinical aspect is a concern. The human amniotic membrane (AM) has antimicrobial effects mostly because of peptides such as alafin and HBDs. Objectives: The aim of this study was to investigate antimicrobial effects of AM on some standard strains of Pseudomonas aeruginosa ATCC27853, Salmonella enterica BAA-708, Escherichia coli ATCC25922, Klebsiella pneumonia ATCC7881, Enterococcus faecalis ATCC29212 and in vivo study on Pseudomonas keratitis. Materials and Methods: AM with and without antibiotic cocktail were prepared from Imam Khomeini hospital of Tehran and cut into 1.5×1.5 cm. The 0.5 McFarland suspension of strains was prepared and cultured on plate media and then both types of membranes were put on cultures. The effect of environmental factors (temperature, time and pH) was considered. In laboratory animal model, keratitis was made in 14 rabbits and they were divided into two control and AM groups. The membrane was bound to the control group. The infiltration wound diameter was measured. Results: The growth inhibition by cocktail membrane occurred in 5 strains, but membrane without cocktail could not inhibit the growth of K. pneumonia and E. faecalis in vitro. Time and pH conferred no antimicrobial effects, while temperature showed an effect on P. aeruginosa. The decrease in the infiltration in the membrane group was significant compared to the control group P<0.05). Conclusion: The AM has antimicrobial properties and can be used alongside antibiotics for the treatment purposes.