Abstract
Introduction: The anti-tumor effects of cisplatin and valproic acid have provided a new
opportunity in the treatment of ovary tumor cell line.
Objectives: In this survey, the cytotoxic and apoptotic effects of cisplatin and valproic acid on
the human ovary tumor cell line (ovar-3) was investigated.
Materials and Methods: In this study three groups were compared with the control group.
Groups 1, 2 and 3 were studied with cisplatin, valproic acid and both of cisplatin and valproic
acid respectively on ovary cell line (ovar-3). Each drug concentration was a spectrum between
1 to 1000 MM. The exposure time of ovar-3 ovary cells with the drugs was 36 hours. The MTT
test for the live cell monitoring was performed with anexinPropidium iodide (PI).
Results: The IC50 for each cisplatin, valproic acid and the mixture of these compounds were 3.3
mm, 33 mm and 0.09 mm, respectively (P<0.05). The morphologic investigation for apoptosis
showed that the mixture of cisplatin and valproic acid has the highest apoptotic effect to a level
of 81% on ovar-3 cell line compared to each of these compounds.
Conclusion: Considering the significant synergistic apoptotic and lower cytotoxic effects of
cisplatin and valproic acid drugs on ovary cells, the mixture of them can be an efficient antitumor
compound targeting for treatment of ovar-3 cell line.