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Submitted: 25 Nov 2024
Revision: 12 Feb 2025
Accepted: 08 Mar 2025
ePublished: 22 May 2025
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Immunopathol Persa. 2025;11(2): 43799.
doi: 10.34172/ipp.2025.43799
  Abstract View: 15
  PDF Download: 12

Original

CD34+CD38-stem cells and CD34+CD38+progenitor cells as markers of chemotherapy response in acute myeloid leukemia patients

Aya Fergany 1* ORCID logo, Khaled M Hassanein 2.3 ORCID logo, Muhamad R Abdel Hameed 4 ORCID logo, Ayat M. Kamel 5 ORCID logo, Asmaa M. Zahran 6 ORCID logo

1 Department of Microbiology and Immunology, Faculty of Pharmacy, New Valley University, New Valley, Egypt
2 Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
3 Department of Microbiology and Immunology, Badr University, Assiut, Egypt
4 Department of Internal Medicine and Hematology Unit, Assiut University Hospitals and Bone Marrow Transplantation Unit, South Egypt Cancer Institute, Assiut University, Assiut, Egypt
5 Department of Microbiology and Immunology, Faculty of Pharmacy, Assiut University, Assiut, Egypt
6 Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt
*Corresponding Author: Aya Fergany, Email: aya-fergany@pha.nvu.edu.eg, Email: ferganyaya81@gmail.com

Abstract

Introduction: CD34+CD38-stem cells were identified as the most related markers to acute myeloid leukemia (AML) progression, resistance, and relapse. However, there is still a lack of published data identifying the level of CD34 and CD38 during induction chemotherapy and after complete remission.

Objectives: This study aimed to evaluate the levels of CD34+CD38+progenitor cells and CD34+CD38-stem cells in AML patients at diagnosis and after induction chemotherapy.

Patients and Methods: This is a prospective cohort study. Both CD34 and CD38 cell markers were identified using flow cytometry in newly diagnosed AML patients and after induction chemotherapy.

Results: Forty newly diagnosed AML patients (27 males and 13 females) were followed up after induction chemotherapy. Results revealed a statistically significant decline (P≤0.05) in CD34+CD38-stem cell levels as well as in CD34+CD38+progenitor cell levels in AML patients who achieved complete or incomplete remission compared to newly diagnosed AML patients. Besides, age and CD34+CD38-stem cells exhibited a statistically significant positive correlation at diagnosis.

Conclusion: Our study showed CD34+CD38-stem cells are associated with disease progression and poor survival in AML patients. We concluded that, CD34 and CD38 are promising follow-up markers for AML patients on induction chemotherapy. In order to keep AML patients in full remission and increase their chances of survival, medications that target leukemic stem cells (LSCs) should be used in conjunction with the usual chemotherapy that targets blasts.


Citation: Fergany A, Hassanein KM, Abdel Hameed MR, Kamel AM, Zahran AM. CD34+CD38-stem cells and CD34 + CD38 + progenitor cells as markers of chemotherapy response in acute myeloid leukemia patients . Immunopathol Persa. 2025;11(2):e43799. DOI:10.34172/ipp.2025.43799.
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