Abstract
Introduction: Coronary artery disease (CAD) is one of the most common causes of mortality throughout worldwide. Metabolic deregulation in peripheral blood mononuclear cells (PBMCs) could play important role in the pathogenesis of atherosclerosis.
Objectives: In the present study, we aim to examine gene expression of legumain and transforming growth factor beta (TGF-β) in patients with significant coronary artery stenosis.
Patients and Methods: In this case control study, 56 CAD participants (percentage of coronary artery stenosis ≥50) and 34 non-CAD participants (percentage of coronary artery stenosis ≤30) were incorporated to the study. Blood sample were collected and PBMCs were isolated. Serum factors were analysed and the percentage of coronary artery stenosis was recorded. The gene expression of legumain and TGF-β was evaluated by real time polymerase chain reaction. Finally, correlation of percentage of coronary artery stenosis with Legumain and TGF-β expression was analyzed.
Results: Legumain and TGF-β gene expression levels had a remarkable correlation with the coronary artery stenosis. Furthermore, fasting serum glucose had a significant positive correlation with the expression of legumain, TGF-β and percentage of coronary artery stenosis.
Conclusion: Alteration of expression of legumain and TGF-β maybe participate to pathogenesis of atherosclerosis.