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Submitted: 15 Nov 2022
Accepted: 20 Jan 2023
ePublished: 16 May 2023
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Immunopathol Persa. 2023;9(2): 39479.
doi: 10.34172/ipp.2023.39479

Scopus ID: 85164622474
  Abstract View: 1141
  PDF Download: 521

Original

Galbanic acid of Ferula assa-foetida L, as a regulator of the AMPK pathway in reduction of lipid accumulation in HepG2 cells

Hadis Musavi 1,2 ORCID logo, Hajar Shokri-Afra 3 ORCID logo, Soleiman Mahjoub 2,4* ORCID logo, Abbas Khonakdar-Tarsi 5,6* ORCID logo, Abouzar Bagheri 5,6 ORCID logo, Zahra Memariani 7 ORCID logo

1 Student Research Committee, Babol University of Medical Sciences, Babol, Iran
2 Department of Clinical Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, Iran
3 Gut and Liver Research Center, Non-communicable Disease Institute, Mazandaran University of Medical Sciences, Sari, Iran
4 Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
5 Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
6 Department of Clinical Biochemistry and Genetics, Faculty of Medicine,Mazandaran University of Medical Sciences, Sari, Iran
7 Traditional Medicine and History of Medical Sciences Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
*Corresponding Authors: Soleiman Mahjoub, Email: smahjoub20@gmail.com, , Email: s.mahjoub@mubabol.ac.ir; Abbas Khonakdar-Tarsi, Email: khonakdarab@gmail.com

Abstract

Introduction: Hepatic fat accumulation is a complication of non-alcoholic fatty liver disease (NAFLD). An AMP-activated protein kinase (AMPK) reduces the synthesis of fatty acids by inhibiting sterol regulatory element-binding transcription factor 1-c (SREBP1-C) and acetyl COA carboxylase (ACC).

Objectives: We aimed to investigate the impress of galbanic acid (Gal) from the Ferula plant on AMPK and its inhibitory effect on lipogenic enzymes in HepG2 cells.

Materials and Methods: In an applied-fundamental study, HepG2 cells were treated for 24 hours with Gal in palmitate (Pal). Resveratrol (RSV) was conducted as a positive control. Fatty acid synthase (FAS) and SREBP1-C gene expression were evaluated by reverse transcription polymerase chain reaction (RT-PCR). FAS, phospho-acetyl-CoA carboxylase (P-ACC), P-AMPK, AMPK, SREBP-1c, and ACC protein levels were measured by western blotting. Lipid accumulation was investigated qualitatively and semi-quantitatively with oil red.

Results: The semi-quantitative results of oil revealed a substantial reduction (P<0.004) in lipid accumulation for treatment with Gal. The significant increase in the protein level of P-AMPK (P<0.001) and P-ACC (P=0.054) and significant decrease in FAS (P<0.003), SREBP-1c (P<0.001) and ACC (P<0.011) due to the effect galbanic acid was observed. FAS gene expression decreased significantly (P<0.009), while the decrease in SREBP-1c gene expression was not significant (P=0.303).

Conclusion: These findings direct that galbanic acid can be a new regulator of AMPK. Hence, the present study may introduce galbanic acid as a new plan to positively regulate the AMPK pathway, which leads to the regulation of various cellular processes.


Citation: Musavi H, Shokri-Afra H, Mahjoub S, Khonakdar-Tarsi A, Bagheri A, Memariani Z. Galbanic acid of Ferula assa-foetida L, as a regulator of the AMPK pathway in reduction of lipid accumulation in HepG2 cells. Immunopathol Persa. 2023;9(2):e39479. DOI:10.34172/ ipp.2023.39479.
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