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Submitted: 01 May 2021
Accepted: 16 Jun 2021
ePublished: 31 Jul 2021
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Immunopathol Persa. 2022;8(1): e4.
doi: 10.34172/ipp.2022.04
  Abstract View: 45
  PDF Download: 19

Original

Cannabis sativa L. oil extract affects neuroinflammation, clinical score, and cannabinoid receptor-1 gene expression in C57bl/6 experimental autoimmune encephalomyelitis

Iman Goraki 1 ORCID logo, Mohammad Reza Shiri-Shahsavar 2,3 ORCID logo, Mohammad Pourhassan-Moghaddam 4 ORCID logo, Sharareh Khangaldi 5 ORCID logo, Sina Khodakarimi 6,9 ORCID logo, Mohammad Ebrahimi-kalan 7 ORCID logo, Mohammad Jafar Maleki 8 ORCID logo, Hanieh Beyrampour-Basmenj 8,9 ORCID logo, Abbas Ebrahimi-kalan 6 * ORCID logo

1 Department of Molecular Biology, Islamic Azad University Ahar Branch, Tabriz, Iran
2 Department of Nutrition, School of Health, Qazvin University of Medical Sciences, Qazvin, Iran
3 Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
4 Institute for Biomedical Materials and Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia
5 Department of Nutrition, School of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
6 Department of Neurosciences and cognition, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
7 Department of Epidemiology, Stempel College of Public Health, Florida International University, Miami, FL, USA
8 Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
9 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
*Correspondence to Abbas Ebrahimi-kalan; Email: Ebrahimiab@tbzmed.ac.ir, Abbasebra@gmail.com

Abstract

Introduction: Multiple sclerosis (MS) is a degenerative central nervous system disease derived by immune mechanisms, which ultimately results in clinical debilities. Numerous nutraceuticals have been cited to be effective in treatment of central nervous system complications.

Objectives: This study investigated the effect of Cannabis sativa L. seed oil on experimental autoimmune encephalomyelitis (EAE).

Materials and Methods: Female C57bl/6 mice were assigned randomly into three groups (8 in each). Group-A received no myelin oligodendrocyte glycoprotein (MOG), group B was immunized by MOG and treated with oil, while in group C animals were immunized and treated with normal saline. Clinical scores were recorded every other day throughout the study and after four weeks, all mice were sacrificed and spinal cords were incised for molecular and histopathological evaluations.

Results: Significant differences were observed in mean clinical scores between control and experiment groups (P<0.001). Cannabinoid receptor-1 gene expression increased significantly in treatment group (P<0.001). Histopathologic evaluations also showed a significant decrease in overall infiltrated and vacuolated area and immune cells infiltration into the central nervous system in the treatment group (P<0.01).

Conclusion: Cannabis sativa L. oil extract administration alleviated inflammation and paralysis in animal model. Therefore, its oil extract might be useful in soothing inflammatory and auto-immune diseases. However, additional research might be required.

Keywords: Cannabis sativa L. oil extract, Neuroinflammation, Histopathology, Cannabinoid receptor-1 gene expression, Multiple sclerosis, Experimental autoimmune encephalomyelitis
Citation: Goraki I, Shiri-Shahsavar M, Pourhassan-Moghaddam M, Khangaldi S, Khodakarimi S, Ebrahimi-kalan M, Maleki M, Beyrampour-Basmenj H, Ebrahimi-kalan A. Cannabis sativa L. oil extract affects neuroinflammation, clinical score, and cannabinoid receptor-1 gene expression in C57bl/6 experimental autoimmune encephalomyelitis. Immunopathol Persa. 2022;8(1):e04. DOI:10.34172/ ipp.2022.04.
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