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Submitted: 28 Mar 2021
Accepted: 03 Aug 2021
ePublished: 26 Aug 2021
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Immunopathol Persa. 2022;8(1): e20.
doi: 10.34172/ipp.2022.20
  Abstract View: 36
  PDF Download: 14

Original

Secretome of adipose derived stem cells induced apoptosis in anaplastic thyroid carcinoma C-643 cells

Fatemeh Rezaei-Tazangi 1 ORCID logo, Ali Samadi 1 * ORCID logo, Saeed Azandeh 2 ORCID logo, Saeed Khoshnood 3 ORCID logo, Shahab Mahmoudvand 4 ORCID logo

1 Department of Basic Sciences, School of Medicine, Bam University of Medical Sciences, Bam, Iran
2 Cellular & Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Clinical Microbiology Research Center, Ilam University of Medical Scienes, Ilam, Iran
4 Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
*Correspondence to Ali Samadi, Ph.D., Email; alisamadi23@mubam.ac.ir

Abstract

Introduction: Previous studies have proven that secretory materials of mesenchymal stem cells (MSCs) (secretome) have remedial properties.

Objectives: The main goal of this study was to evaluate the effects of secretome of adipose-derived MSCs on growth and apoptosis in anaplastic thyroid carcinoma (ATC) C-643 cells.

Materials and Methods: Initially, thyroid carcinoma cells were exposed to the 25 and 50 μg/mL adipose-derived stem cells (ADSCs) secretome for 24 and 48 hours, to evaluate the proliferation and cytotoxicity of the C-643 cells, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test and colony assay was conducted. Acridine orange/ethidium bromide (AO/EB) staining was conducted to evaluate the apoptosis. The expression level of apoptosis-associated genes was determined by real-time polymerase chain reaction (PCR) technique.

Results: Cell viability and colony numbers in groups exposed to secretome were significantly lower than the control group. The amount of apoptosis-related genes (Bax/Bcl-2, P53, caspase-3 and caspase-8) expression in secretome-treated groups was more than the control group.

Conclusion: The data revealed that ADSCs secretome caused the significant reduction of cell growth and induced apoptosis in C-643 cells.


Keywords: Mesenchymal stem cells, Secretome, Thyroid carcinoma, Viability, Apoptosis
Citation: Rezaei-Tazangi F, Samadi A, Azandeh S, Khoshnood S, Mahmoudvand S. Secretome of adipose derived stem cells induced apoptosis in anaplastic thyroid carcinoma C-643 cells. Immunopathol Persa. 2022;8(1):e20. DOI:10.34172/ ipp.2022.20.
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