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Submitted: 20 Dec 2019
Accepted: 07 Jan 2020
ePublished: 06 May 2020
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Immunopathol Persa. 2020;6(2): e21.
doi: 10.34172/ipp.2020.21
  Abstract View: 273
  PDF Download: 122

Original

Relation between Immune cell response and stemness genes expression in breast cancer; A new approach in NANOG gene and Let7-a expression in breast cancer cell lines

Zeynab Aliyari-Serej 1,2,3 ORCID logo, Ayyub Ebrahimi 4 ORCID logo, Tohid Kazemi 2,5 ORCID logo, Souzan Najafi 2, Elmira Roshani 2, Mohammad Ebrahimi-Kalan 6 ORCID logo, Behzad Baradaran 2,5 * ORCID logo

1 Department of Applied Cell Sciences, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
2 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Halic Uuniversity, Istanbul, Turkey
5 Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
6 Department of Epidemiology, Stempel College of Public Health, Florida International University, Miami, FL, USA
*Correspondence to Behzad Baradaran, Email: behzad_im@yahoo.com , baradaranb@tbzmed.ac.ir

Abstract

Introduction: Failure and recurrence in breast cancer treatment cause a great obstacle in cancer therapy and identification of cell population named cancer stem cells (CSCs) in the tumor can be led us to define it as target in novel therapeutic strategy.

Objectives: The aim of this study is the finding of correlation between stemness and metastatic characteristic, also knowing CSCs as a potential target of therapy because of its developmental behavior and similarities with normal stem cells.

Materials and Methods: Here, we focus on the expression of NANOG in breast CSCs, a key molecule in the physiological process of stem cells and the Let-7a that is involved in the differentiation of the cells.

Results: In this work, we found that NANOG was highly expressed in SKBR3 and down-regulation of let-7a, as a differentiation miRNA, was found in MDA-MB-468 cells.

Conclusion: It will be critical for the developing of effective anti-tumor drugs, utilizing mentioned concepts. Inhibition of NANOG in combination with Let-7a up-regulation can help to decrease the stemness and increase the differentiation of CSCs. The decrease of stemness and increase of differentiation initiate the apoptotic process. So, modification in the mechanism of apoptosis beside anti-cancer drugs provide a good preclinical study goal. However, in order to these drugs become clinical, the problems of their side effects and toxicity must be solved. Differentiation of CSCs provides an optimal condition to activity of immune cells which never let them escape from immune cells by alteration of immunogenicity.

Keywords: Breast cancer, Immunologic response, Stemness, Cancer stem cells, Novel cancer therapy
Citation: Aliyari-Serej Z, Ebrahimi A, Kazemi T, Najafi S, Roshani E, Ebrahimi-Kalan M, et al. Relation between Immune cell response and stemness genes expression in breast cancer; A new approach in NANOG gene and Let7-a expression in breast cancer cell lines. Immunopathol Persa. 2020;6(2):e21. DOI:10.34172/ ipp.2020.21.
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