Submitted: 13 Jan 2018
Accepted: 06 May 2018
First published online: 15 May 2018
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Immunopathol Persa. 2018;4(2):e25-e25.
doi: 10.15171/ipp.2018.25
  Abstract View: 142
  PDF Download: 376

Original

Antimicrobial effects of amniotic membrane on some bacterial strains

Siamak Heidarzadeh 1, Abdolmajid Ghasemian 2,3, Zohreh Kalafi 4, Farhad Nikkhahi 1, Mohammad Mehdi Soltan Dallal 1,4 *

1 Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Microbiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3 Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran
4 Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
*Correspondence to Mohammad Mehdi Soltan Dallal, Email: Email: msoltandallal@gmail.com

Abstract

Introduction: Antibiotic resistance in clinical aspect is a concern. The human amniotic membrane (AM) has antimicrobial effects mostly because of peptides such as alafin and HBDs.

Objectives: The aim of this study was to investigate antimicrobial effects of AM on some standard strains of Pseudomonas aeruginosa ATCC27853, Salmonella enterica BAA-708, Escherichia coli ATCC25922, Klebsiella pneumonia ATCC7881, Enterococcus faecalis ATCC29212 and in vivo study on Pseudomonas keratitis.

Materials and Methods: AM with and without antibiotic cocktail were prepared from Imam Khomeini hospital of Tehran and cut into 1.5×1.5 cm. The 0.5 McFarland suspension of strains was prepared and cultured on plate media and then both types of membranes were put on cultures. The effect of environmental factors (temperature, time and pH) was considered. In laboratory animal model, keratitis was made in 14 rabbits and they were divided into two control and AM groups. The membrane was bound to the control group. The infiltration wound diameter was measured.

Results: The growth inhibition by cocktail membrane occurred in 5 strains, but membrane without cocktail could not inhibit the growth of K. pneumonia and E. faecalis in vitro. Time and pH conferred no antimicrobial effects, while temperature showed an effect on P. aeruginosa. The decrease in the infiltration in the membrane group was significant compared to the control group P<0.05).

Conclusion: The AM has antimicrobial properties and can be used alongside antibiotics for the treatment purposes.

Citation: Heidarzadeh S, Ghasemian A, Kalafi Z, Nikkhahi F, Soltan Dallal MM. Antimicrobial effects of amniotic membrane on some bacterial strains. Immunopathol Persa. 2018;4(2):e25. DOI:10.15171/ ipp.2018.25.
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